Table 1. Rate constants obtained with p- and s-polarized light for the reconstitution of CCR5 StaR into a lipid membrane composed of PC, PC/chol (8/2 mol/mol) and PC/SM/Chol (6/2/2 mol/mol/mol).
| Polarization |
p-pol |
s-pol |
||
|---|---|---|---|---|
| Phase | Fast (sec−1) | Slow (sec−1) | Fast (sec−1) | Slow (sec−1) |
| POPC | 3.8e−3 ± 8.8e−4 | 3.3e−4 ± 5.9e−5 | 5.5e−3 ± 9.1e−4 | 3.3e−4 ± 7.5e−5 |
| POPC/Chol (8/2) | 2.4e−3 ± 4.3e−4 | 3.0e−4 ± 1.1e−4 | 4.2e−3 ± 3.2e−4 | 3.1e−4 ± 5.8e−5 |
| POPC/SM/Chol (6/2/2) | 5.7e−3 ± 5.7e−4 | 7.6e−4 ± 6.5e−5 | 9.0e−3 ± 2.9e−4 | 8.8e−4 ± 9.3e−5 |
The reconstitution process follows a two-stage exponential process with two rate constants associated (fast and slow; see Materials and Methods for details). The mean value obtained from at least 4 different experiments and associated standard deviation is provided (95% confidential intervals). Significativity between the different values is provided in SI. Data is presented in the form of bars in Fig. 3.