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. 2016 Nov 8;6:36174. doi: 10.1038/srep36174

Figure 2. Influence of a GFP-derived linker on trafficking.

Figure 2

Left panel shows structure of expressed PfHsp70x-GFP chimeras and live cell imaging of transfectants, right shows Streptolysin O fractionation followed by western blot using the antibodies indicated. DIC, differential interference contrast; in merge and overlay blue, Hoechst (nuclear stain); green, GFP; S, supernatant following SLO fractionation; P, pellet following SLO fractionation. Size markers in kDa. Pictures are representative of at least 10 individual observations, western blots of at least 3 independent experiments. The lower GFP band in the pellet fraction is a commonly observed GFP degradation product.