Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Nov 8;6:36174. doi: 10.1038/srep36174

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

Left panel shows structure of expressed PfHsp70x-GFP chimeras and live cell imaging of transfectants, right shows Streptolysin O fractionation followed by western blot using the antibodies indicated. Mutated residues are shown in light green, original motif in bold. Red indicates the first 25 amino acids of PfHsp70x, predicted signal sequence. Numbering refers to position in motif following signal sequence cleavage. DIC, differential interference contrast; in merge and overlay blue, Hoechst (nuclear stain); green, GFP; S, supernatant following SLO fractionation; P, pellet following SLO fractionation. Size markers in kDa. Pictures are representative of at least 10 individual observations, western blots of at least 3 independent experiments. The lower GFP band in the pellet fraction is a commonly observed GFP degradation product.