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. 2016 Nov 8;6:36239. doi: 10.1038/srep36239

Figure 4. Cytotoxicity assay using HUVEC, REH and MOLT-4 cell lines.

Figure 4

(A) Relative survival using EcASNase2. (B) Relative survival using ScASNase1. Cells were incubated with 10 U/mL of ScASNase1 or EcASNase2 (Prospec–Tany, Israel) for 72 h. The control is represented by the cell culture containing only RPMI 1640 medium with 10% foetal bovine serum. The enzyme buffer was Tris–HCl 50 mM pH 8.8 to ScASNase1 or Tris–HCl 50 mM pH 7.4 to EcASNase2. Error bars were calculated from the mean ± SD (n = 3). Statistical analysis using the ANOVA with Bonferroni test performed using GraphPad Prism 6.05 software showed p < 0.001 versus respective control and buffer.