Table 1. Kinetic parameters of ScASNase1 to hydrolyse of L-asparagine and comparison with EcASNases.
| EcASNase2 | Km (μM) | Vmax (μmol/min) | kcat (s−1) | kcat/Km (M−1.s−1) | nH |
|---|---|---|---|---|---|
| L-asparagine | 23 ± 5 | 0.032 ± 0.00061 | 134 ± 2 | 5.8 ×106 | 1.1 ± 0.1 |
| ScASNase1 | K0.5(μM) | Vmax (μmol/min) | kcat (s−1) | kcat/[K0,5] nH (M−1. s−1) | nH |
| L-asparagine | 75 ± 27 | 0.042 ± 0.0011 | 217 ± 14 | 1.6 × 104 | 2.2 ± 0.3 |
| L-asparagine +L-glutamine | 122 ± 31 | 0.097 ± 0.0023 | 523 ± 34 | 1.3 × 104 | 2.2 ± 0.2 |
| EcASNase1a* | |||||
| L-asparagine | 400 ± 50 | − | 7.4 ± 0.3 | 5.8 × 10−3 | 3.5 ± 0.3 |
a*Kinetics data from Karamitros and Konrad, 2014 – using the same NAD+ coupled assay29.