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. 2016 Nov 8;6:36266. doi: 10.1038/srep36266

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Copyright © 2016, The Author(s)

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(A) Phase contrast microscopy for human OMECs seeded on AMs after isolation. Close-up views of selected areas in the photo of cells at Day 14 are indicated by square brackets, whereas approximate cell size is indicated by a dotted red line. Human OMECs isolated by collagenase exhibited a more compact morphology. The bars represent 100 μm. (B) In oral mucosal tissue, cytokeratin 3/76 (K3/76; green) was expressed in non-keratinized epithelia, whereas cytokeratin 8 (K8; green) was not expressed. When human OMECs were cultivated on the AM surface for 14 days, similar K3/76-positive and K8-negative staining patterns were exhibited in both the collagenase (Col.) or dispase II/trypsin (D/T) treated groups. The bars represent 75 μm.