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. 2016 Oct 3;67(21):5975–5991. doi: 10.1093/jxb/erw352

Fig. 3.

Fig. 3.

Expression analysis of selected gibberellin (GA) pathway genes during axillary bud (AXB) development under long and short days, and after stem decapitation. (A) GA-deactivating GA2-oxidase-like genes, GA2ox1, GA2ox3, GA2ox4, and GA2ox5. (B) GA biosynthesis genes, belonging to GA3-oxidase (GA3ox1 and GA3ox2) and GA20-oxidase families (GA20ox4 and GA20ox8). (C) DELLA-like gene (DE1) and two GID1-receptor-like genes (GID1A and GID1B). (A–C) Fold change under long days in the apex and AXBs (LD; blue dots and lines) and after 5 weeks of short days (SD; open circles, stippled lines) for the terminal bud and AXBs up to node 14. The red dot indicates the expression level in AXB 12 of intact plants, at the bud maturation point (BMP). After stem decapitation at the BMP, gene expression was measured at day 8 in five successive AXBs (1–5) directly proximal to the cut (A–C, insets) (x-axis fold change), and (D) during a 7 d period in AXB 12, proximal to the cut. The values (A–C) are calculated relative to the apex expression level (set at 1), and in the inset relative to each individual AXB position before decapitation (set at 1, red line) and (D) relative to the AXB position 12 before decapitation. Values represent means of six plants ±SE, analyzed in two pooled samples.