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. 2016 Oct 17;67(21):6111–6123. doi: 10.1093/jxb/erw373

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© The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 1. — FIE protein accumulates in all Arabidopsis tissues and organs. (A) Detection of endogenous FIE protein in Arabidopsis tissues. A similar amount of nuclear chromatin protein extracts from rosette, stems, cauline, and inflorescence of 20-day-old wild-type plants and siliques from 6–10 days after pollination (DAP) were separated by SDS-PAGE and then immunodetected using αFIE antibodies. Protein size marker is indicated on the left. Ponceau staining was used to assess equal sample loading. (B) A scheme of the ProFIE:FIE_gDNA-GFP transgene construct (not drawn to scale). Red boxes represent exons. (C) gFIE-GFP chimeric protein from line no. TH142-1 is observed in reproductive and vegetative tissues and organs. Each image was taken from a single focal plane, unless otherwise indicated. Each panel shows a merge of GFP epifluorescence (green) and the corresponding chloroplast autofluorescence (red) and/or a bright-field image. (1) Rosette leaf, abaxial epidermis; scale bar (SB): 50 μm. (2) Rosette leaf, mesophyll cells; SB: 25 μm. (3) Trichome on the surface of a rosette leaf, Z-stack overlay; SB: 50 μm. (4) Hypocotyl, Z-stack overlay; SB: 50 μm. (5) Cauline leaf, abaxial epidermis; SB: 25 μm. (6) Petal; SB: 25 μm. (7) Sepal; SB: 50 μm. (8) Carpel; SB: 250 μm. (9) Pollen; SB: 20 μm. (10) Unfertilized ovules; SB: 50 μm. (11) Developing seed containing a four cell-stage embryo; SB: 50 μm. (12) Developing seed containing a heart-stage embryo; SB: 100 μm. (13) Main root, root hairs; SB: 50 μm. (14) Main and lateral roots; SB: 500 μm. (15) Budding lateral root; SB: 50 μm. (16) Main root tip, quiescent center; SB: 50 μm.