Table 1.
Nuclear and Mitochondrial Genotypes of Cybrid Lines.
| Line (Rep)a | P0 strains | Line nuclear genotypec | Line mitochondrial genotypee | |||
|---|---|---|---|---|---|---|
| Maternal | Paternal | Expb | Obs | Expd | Obsf | |
| RC-PH (1) | HK105 | PB800 | Te | – | HK105 | HK105 |
| RC-PH (2) | HK105 | PB800 | Te | – | HK105 | F |
| RC-PH (3) | HK105 | PB800 | Te | – | HK105 | PB800 |
| RC-HP (1) | PB800 | HK105 | Te | – | PB800 | PB800 |
| RC-HP (2) | PB800 | HK105 | Te | – | PB800 | PB800 |
| RC-HP (3) | PB800 | HK105 | Te | – | PB800 | PB800 |
| RC-EH (1) | HK104 | EG4818 | Te | – | HK104 | EG4181 |
| RC-EH (2) | HK104 | EG4818 | Te | – | HK104 | HK104 |
| RC-EH (3) | HK104 | EG4818 | Te | – | HK104 | EG4181 |
| RC-HE (1) | EG4818 | HK104 | Te | – | EG4818 | HK104 |
| RC-HE (2) | EG4818 | HK104 | Te | – | EG4818 | HK104 |
| RC-HE (3) | EG4818 | HK104 | Te | – | EG4818 | HK104 |
| MR-AH (1) | HK105 | AF16 | Tr | Tr | HK105 | HK105 |
| MR-AH (2) | HK105 | AF16 | Tr | Tr | HK105 | AF16 |
| MR-AH (3) | HK105 | AF16 | Tr | Tr | HK105 | HK105 |
| MR-AD (1) | DL232 | AF16 | Tr | Tr | DL232 | DL232 |
| MR-AD (2) | DL232 | AF16 | Tr | Tr | DL232 | DL232 |
| MR-AD (3) | DL232 | AF16 | Tr | Tr | DL232 | DL232 |
| MR-AE (1) | ED3101 | AF16 | Tr | Tr | ED3101 | ED3101 |
| MR-AE (2) | ED3101 | AF16 | Tr | Tr | ED3101 | ED3101 |
| MR-AE (3) | ED3101 | AF16 | Tr | Tr | ED3101 | ED3101 |
| MR-AJ4 (1) | JU403 | AF16 | Tr | Tr | JU403 | JU403 |
| MR-AJ4 (2) | JU403 | AF16 | Tr | Tr | JU403 | JU403 |
| MR-AJ4 (3) | JU403 | AF16 | Tr | Tr | JU403 | JU403 |
| MR-AJ1 (1) | JU1345 | AF16 | Tr | Tr | JU1345 | JU1345 |
| MR-AJ1 (2) | JU1345 | AF16 | Tr | Tr | JU1345 | JU1345 |
| MR-AJ1 (3) | JU1345 | AF16 | Tr | Tr | JU1345 | JU1345 |
| MR-AP (1) | PB800 | AF16 | Tr | Tr | PB800 | PB800 |
| MR-AP (2) | PB800 | AF16 | Tr | Tr | PB800 | PB800 |
| MR-AP (3) | PB800 | AF16 | Tr | Tr | PB800 | PB800 |
| MR-AV (1) | VT847 | AF16 | Tr | – | VT847 | VT847 |
| MR-AV (2) | VT847 | AF16 | Tr | – | VT847 | VT847 |
| MR-AV (3) | VT847 | AF16 | Tr | – | VT847 | VT847 |
| CP129 (1) | HK104 | AF16 | AF16 | AF16 | HK104 | HK104 |
| CP130 (2) | HK104 | AF16 | AF16 | AF16 | HK104 | HK104 |
| CP131 (1) | AF16 | HK104 | HK104 | HK104 | AF16 | AF16 |
| CP132 (2) | AF16 | HK104 | HK104 | HK104 | AF16 | HK104 |
| CP133 (3) | AF16 | HK104 | HK104 | HK104 | AF16 | AF16 |
aAll crosses were replicated “(Rep)” thrice, with the exception of AF16 male×HK104 hermaphrodite (producing CP129 and CP130), for which one of three lines initiated went extinct.
bThe expected nuclear genotype is the P0 male wild isolate genotype.
cIsolates belong to the tropical (“Tr”), temperate (“Te”) or equatorial Kenya phylogenetic clade (Cutter et al. 2010). Nuclear genotype was observed either by an AFLP that distinguishes temperate from tropical alleles or at five loci distinguishing AF16 and HK104 (CP129–CP133). Because the former assays only distinguish members of different clades, nuclear genotypes of within-clade (Te×Te or Tr×Tr) hybrids were unable to be obtained (–).
dThe expected mitochondrial genotype is the P0 maternal wild isolate genotype.
eMitochondrial genotype was observed either by sequencing the COII gene or by an RFLP distinguishing the AF16 and HK104 mitotypes (CP129–CP133).
fThe observed and expected mitotypes occasionally did not match (gray shading), providing evidence for paternal mitochondrial transmission.
F, failed PCR reaction.