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. 2016 Nov 8;11:128. doi: 10.1186/s13000-016-0580-5

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Representative images of venous and lymphatic invasion. Legend: a, b To determine the presence or absence of venous invasion, both EVG staining and immunohistochemistry for CD31 were performed. Venous invasion was confirmed, but no CD31-positive cells were identified. Such difficult cases were reviewed by more than two expert pathologists (EVG staining and immunohistochemistry for CD31; original magnification, ×200; scale bars represent 200 μm). c To determine the presence or absence of lymphatic invasion, immunohistochemistry for D2-40 was performed. Difficult cases were examined by more than two expert pathologists (Immunohistochemistry for D2-40; original magnification × 400; scale bar represents 100 μm)