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. 2016 Nov 8;16:262. doi: 10.1186/s12866-016-0878-1

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Competitive biofilm experiment. MC1000 and BP19 were grown at a starting ratio of 1:1. Biofilm bound bacteria were enumerated on LB and LB/kn plates. The total number of bacteria (white diamonds, dashed line) is presented as colony forming units per well (CFU/well) and was calculated from the LB plates. To determine the percentage of flhD mutants (black squares, solid line), bacterial counts from the kanamycin plates were divided by total bacterial numbers. Dashed lines mark 50 and 100 % flhD mutants