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. 2016 Nov 8;16:869. doi: 10.1186/s12885-016-2909-6

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Knockdown of sFRP2 in metastatic mouse cells decreases in vitro migratory and invasive potential for OS. a CCK-8 cell proliferation assay of multiple shsFRP2/RF1044 and RF1044 vector control cells. Western blot analysis showing expression of sFRP2 in control and knockdown cells. b Cell migration assay of shsFRP2/RF1044 and control RF1044 cells. c Transwell invasion assay of control RF1044 (left panel) and shsFRP2/RF1044 (right panel) cells. Crystal violet staining for control and shsFRP2 expressing cells is shown. Quantification of tumor cell invasion is shown to the right. d 3D matrigel assay for vector control (top panel) and shsFRP2/RF1044 cells (bottom panel)