Fig. 2.

IgG N-glycan hydrolyzing activity can be detected during growth of C. pseudotuberculosis. a. C. pseudotuberculosis was grown in 100 ml BHI. After 4 h, 500 μl of human plasma was added to the culture. 1 ml samples were taken from the bacterial culture after 8, 10,12, 24 h. IgG was purified from the culture and IgG glycan hydrolysis was analyzed by SDS-PAGE and LCA blot. b. The OD of bacterial culture was measured at 600 nm at the indicated time point