Fig. 3.

CP40 hydrolyzes N-linked glycans on human and animal IgG. a. Recombinant CP40 (0.5 μg) was incubated with to 1 μg of human. As controls, 0.5 μg of MBP and PNGases F were incubated with human IgG. b. ovine, equine, bovine and caprine IgG (1 μg) were incubated with 0.5 μg of CP40 and EndoS. c. All subclasses of human IgG (1 μg) were incubated with 0.5 μg of recombinant CP40. All of the reactions were at 37 °C for 2 h and IgG glycan hydrolyzing was analyzed by SDS-PAGE and LCA blot