Fig. 4.

CP40 is not a general chitinase and does not show glycosidase activity on several glycoproteins. a. CP40, EndoSd, MBP and a chitinase from S. griseus were incubated with the fluorescent substrate 4MU-GlcNAc for 1 h and fluorescence was measured at 355/460 nm. The experiments were performed in five replicates. Data are presented as mean ± S.D. The absorbance was analyzed statistically by One-way Anova followed up by Tukey’s multiple comparisons test. ****P < 0.0001. b. Recombinant CP40 (0.5 μg) was incubated with fetuin, IgE, IgA, IgD, IgM, AGP, hLF for 2 h at 37 °C and glycan hydrolyzing activity were analyzed by SDS-PAGE