Fig. 5.

OLFML2A and OLFML2B double stained immunoreactivity in baboon retina. a Negative Control of baboon retina sections wherein the incubation with the 1st Ab is omitted and incubated with only 2nd Ab: goat anti-rabbit IgG-Cy3® 1:4000. b Confocal images of double stained retina sections to identify cells expressing OLFML2A (red, 1st Ab: rabbit polyclonal anti-human OLFML2A 1:500; 2nd Ab: goat anti-rabbit IgG-Cy3® 1:4000) and β-Tubulin (green, 1st Ab: mouse monoclonal anti-mammal Tubulin 3 beta chain 1:250) in baboon retina. c Confocal images of double stained retina sections to identify cells expressing OLFML2A (red, 1st Ab: rabbit polyclonal anti-human OLFML2A 1:500; 2nd Ab: goat anti-rabbit IgG-Cy3® 1:4000) and Glial Fibrillary Acid Protein (green, 1st Ab: mouse monoclonal anti-GFAP 1:300, 2nd Ab: goat anti mouse IgG FITC 1:250) in baboon retina. Cells nuclei were labeled with DAPI (blue). GCL ganglion cell layer, INL inner nuclear layer, ONL outer nuclear layer, PL photoreceptor layer