Table 2.
The primers used for quantitative PCR
| Gene | Forward primer (5' to 3') | Reverse primer (5' to 3') |
|---|---|---|
| 36B4 | GGCCCTGCACTCTCGCTTTC | TGCCAGGACGCGCTTGT |
| Adiponectin | AAGAAGGACAAGGCCGTTCTCTT | GCTATGGGTAGTTGCAGTCAGTT |
| Arg-1 | CTCCAAGCCAAAGTCCTTAGAG | AGGAGCTGTCATTAGGGACATC |
| BDH | AGTTTGGGGTCGAGGCTTTC | TGGTGGCCGCTATGAAGTTG |
| CD36 | AATGGCACAGACGCAGCCT | GGTTGTCTGGATTCTGGA |
| CD68antigen | TGACCTGCTCTCTCTAAGGCTACA | TCACGGTTGCAAGAGAAACATG |
| CD137 | CGTGCAGAACTCCTGTGATAAC | GTCCACCTATGCTGGAGAAGG |
| D2 | GCACGTCTCCAATCCTGAAT | TGAACCAAAGTTGACCACCA |
| Elovl6 | TTCCGAGTCTCCCGGAAGT | ACAGCCCATCAGCATCTGAGT |
| F4/80 | TGACAACCAGACGGCTTGTG | GCAGGCGAGGAAAAGATAGTGT |
| FAS | GCTGCGGAAACTTCAGGAAAT | AGAGACGTGTCACTCCTGGACTT |
| FGF21 | ATGGAATGGATGAGATCTAGAGTTGG | TCTTGGTCGTCATCTGTGTAGAGG |
| GRP120 | GCATAGGAGAAATCTCATGG | GAGTTGGCAAACGTGAAGGC |
| HADH | ACTACATCAAAATGGGCTCTCAG | AGCAGAAATGGAATGCGGACC |
| HMGCS2 | ATCAACTCCCTGTGCCTGAC | GCAATGTCACCACAGACCAC |
| Leptin | GACACCAAAACCCTCAT | CAGAGTCTGGTCCATCT |
| MCAD | GATCGCAATGGGTGCTTTTGATAGAA | AGCTGATTGGCAATGTCTCCAGCAAA |
| MCP-1 | CTTCTGGGCCTGCTGTTCA | CCAGCCTACTCATTGGGATCA |
| Mincle | ACCAAATCGCCTGCATCC | CACTTGGGAGTTTTTGAAGCATC |
| MR | CCACAGCATTGAGGAGTTTG | ACAGCTCATCATTTGGCTCA |
| NOS2 | CAGCTGGGCTGTACAAACCTT | CATTGGAAGTGAAGCGTTTCG |
| PGC1α | AAGTGTGGAACTCTCTGGAACTG | GGGTTATCTTGGTTGGCTTTATG |
| PPARα | CCTCAGGGTACCACTACGGAGT | GCCGAATAGTTCGCCGAA |
| PPARγ1 | GAGTGTGACGACAAGATTTG | GGTGGGCCAGAATGGCATCT |
| PPARγ2 | TCTGGGAGATTCTCCTGTTGA | GGTGGGCCAGAATGGCATCT |
| SCD1 | CCCCTGCGGATCTTCCTTAT | AGGGTCGGCGTGTGTTTCT |
| SREBP-1c | GGAGCCATGGATTGCACATT | CCTGTCTCACCCCCAGCATA |
| TBX1 | GGCAGGCAGACGAATGTTC | TTGTCATCTACGGGCACAAAG |
| TMEM26 | ACCCTGTCATCCCACAGAG | TGTTTGGTGGAGTCCTAAGGTC |
| UCP1 | GGCCCTTGTAAACAACAAAATAC | GGCAACAAGAGCTGACAGTAAAT |
| UCP2 | ACCAAGGGCTCAGAGCATGCA | TGGCTTTCAGGAGAGTATCTTTG |
36B4 acidic ribosomal phosphoprotein P0, Arg arginase, BDH 3-hydroxybutyrate dehydrogenase, CD36 fatty acid translocase, D2 type 2 iodothyronine deiodinase, Elovl6 elongation of very long-chain fatty acid 6, FAS fatty acid synthase, FGF21 fibroblast growth factor 21, GPR G-protein-coupled receptor, HADH hydroxyacyl-coenzyme A dehydrogenase, HMGCS2 3-hydroxy-3-methylglutaryl-coenzyme A synthase 2, MCAD medium-chain acyl-CoA dehydrogenase, MCP monocyte chemoattractant protein, Mincle the macrophage inducible C-type lectin, MR mannose receptor, NOS nitric oxide synthase, PGC peroxisome proliferator-activated receptor γ coactivator, PPAR peroxisome proliferator-activated receptor, SCD1 stearoyl-CoA desaturase 1, SREBP sterol regulatory element-binding protein, TBX1 T-box 1 transcription factor C, TMEM26 transmembrane protein 26, UCP uncoupling protein