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. 2016 Nov 7;215(3):383–399. doi: 10.1083/jcb.201603080

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Figure 1. — SIM imaging reveals concentric actomyosin arcs in the pSMAC region of the Jurkat T cell IS. (A, first and second panel) 3D-SIM image of an activated Jurkat T cell stained with phalloidin. (A, third panel) Cell in first panel color-coded by z position. Lighter colors are closer to the coverslip. (B) 3D-SIM image of a Jurkat T cell stained with phalloidin (red) and anti–myosin IIA antibody (green). Individual channels and the merged image are shown. (C) Still images from a TIRF-SIM video of a Jurkat T cell expressing GFP–F-Tractin (Video 1). (D) Still images from two color TIRF-SIM videos of two different Jurkat T cells expressing GFP–myosin IIA and tdTomato–F-Tractin (Video 2, corresponding to third panel). SMAC zones bracketed in A–C at top. Bars, 5 µm.