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. 2016 Nov 7;215(3):383–399. doi: 10.1083/jcb.201603080

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Figure 4. — Arp2/3 inhibition augments the assembly of the formin-nucleated linear actin filaments in the dSMAC. (A) Still images from a confocal video of a Jurkat T cell expressing GFP–F-Tractin before (left) and 3 min after (middle) treatment with 25 µM CK666 and after drug washout (right; and Video 5). (B) 3D-SIM images of phalloidin-stained Jurkat T cells treated with 25 µM CK666 for the indicated times. (C) Still images from a TIRF-SIM video of a Jurkat T cell expressing GFP–F-Tractin treated with CK666 as in B (Video 6). (D, top) 3D-SIM images of Jurkats treated with DMSO, 25 µM CK666, or 25 µM CK666 plus 10 µM SMIFH2 and stained with phalloidin. The red bracket in the second panel marks a spike. (D, bottom) Quantitation of mean length of linear actin spikes per cell (left) and their surface density (right). n = 16–22 cells/condition. In B and C, yellow arrowheads mark linear actin spikes, and red arrowheads mark their bend points. Data are means ± SD. Bars, 5 µm. ****, P < 0.0001.