Figure 9.
Actin arcs propel the centripetal transport of TCR MCs across the pSMAC. (A and B) Still images from two separate TIRF-SIM videos of Jurkat T cells expressing GFP–F-Tractin (grayscale) stimulated on planar lipid bilayers containing Alexa Fluor 568–labeled anti-CD3 antibody (red) to report TCR MC movement (Videos 9 and 10). (C) Quantitation of rates of centripetal movement of actin and TCR MCs across the dSMAC and pSMAC obtained from Videos 9 and 10. n = 15–19 measurements/condition. Box plots are centered on means and display upper and lower quartile ranges and min to max values. Bars, 1 µm.