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. 2016 Nov 7;215(3):357–368. doi: 10.1083/jcb.201604118

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© 2016 Moriwaki and Goshima

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 1. — CLASP^Mast/Orbit slows growth and inhibits catastrophe in vitro. (A) Schematic of in vitro MT polymerization assay used in this study. Green, tubulin; orange, biotinylation; light blue, anti-biotin antibody; red/yellow/blue, recombinant proteins. (B) CLASP^Mast/Orbit slows MT growth and inhibits catastrophe. Kymographs show GFP-CLASP^Mast/Orbit (wild type [WT] and TOG domain mutant [TOGmut]) and MT (10 µM S2 tubulin). (C and D) MT growth rate and catastrophe frequency in the presence of 45 nM CLASP^Mast/Orbit (wild type and TOG domain mutant) and 10 µM S2 tubulin. The mean values of each experiment are marked in gray, whereas the mean values of all the experiments are indicated by black columns (two independent experiments). Actual values are plotted in these graphs (n = 15–22 MTs). Bars: (horizontal) 10 µm; (vertical) 2 min.