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. 2016 Nov 7;215(3):357–368. doi: 10.1083/jcb.201604118

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© 2016 Moriwaki and Goshima

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Figure 3. — Effect of Sentin or kinesin-13^Klp10A removal from the MT polymerization reaction. (A) MTs dramatically increased because of reduced catastrophe frequency in the absence of kinesin-13^Klp10A. The other four factors were mixed with 15 µM S2 tubulin for 20 min. Bar, 10 µm. (B–G) MT polymerization dynamics in the presence or absence of Sentin. The other four factors (EB1, XMAP215^Msps, kinesin-13^Klp10A, and 15 nM CLASP^Mast/Orbit) were mixed with 15 µM S2 tubulin (three independent experiments). Actual values are presented in Table S1. Bars: (horizontal) 5 µm; (vertical) 2 min.