Table 1. Kinetic parameters of in vitro–reconstituted MT polymerization dynamics with the addition of five factors.
| Condition | CLASP concentration | n | Growth rate | Shrinkage rate | Catastrophe frequency | Rescue frequency |
|---|---|---|---|---|---|---|
| nM | µm/min | µm/min | ×10−3 s−1 | ×10−3 s−1 | ||
| Tubulin alone | 0 | 3 | 1.6 ± 0.1 (60) | 37.9 ± 8.2 (30) | 0.9 ± 0.2 (31) | 10.5 ± 6.1 (6) |
| Plus EB1/Sentin/Msps/Klp10A/CLASP | 0 | 3 | 5.8 ± 2.2 (88) | 19.0 ± 3.6 (86) | 17.7 ± 2.0 (86) | 5.3 ± 5.8 (7) |
| 15 | 3 | 4.2 ± 1.3 (260) | 9.1 ± 2.5 (219) | 13.9 ± 3.6 (217) | 81.6 ± 11.2 (211) | |
| 45 | 3 | 3.0 ± 1.0 (166) | 5.6 ± 0.9 (88) | 5.7 ± 3.9 (86) | 86.1 ± 23.9 (87) | |
| P (CLASP 45 nM vs. 0 nM) | 0.116 (n = 3) | 0.003 (n = 3) | 0.009 (n = 3) | 0.005 (n = 3) | ||
| 0.002 (exp1) | <10−4 (exp1) | |||||
| <10−4 (exp2) | <10−4 (exp2) | |||||
| <10−4 (exp3) | <10−4 (exp3) |
Values are mean ± SD of three independent experiments (total number of events). See Fig. 2 for a graphic presentation of these results.