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. 2016 Nov 7;215(3):345–355. doi: 10.1083/jcb.201606042

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© 2016 Ulmschneider et al.

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Figure 3. — Increasing pHi promotes excessive FC differentiation. (A) Overexpression of DNhe2 (gray boxes) increases pHi relative to controls (white boxes) in pFCs and FCs. n = 15–17 germaria; n = 3 independent replicates. (B) Penetrance of the stalk phenotypes with overexpression of DNhe2 or RNAi knockdown of CG8177. n > 100 for all genotypes; n > 3 independent replicates. (C–E) Overexpression of DNhe2 or knockdown of CG8177 causes multilayered stalks. Stalk cells in a wild-type ovariole (C) form a single row, whereas overexpression of DNhe2 (D) or knockdown of CG8177 (E) causes the formation of multilayered stalks (white arrows). Insets are enlarged in C–E (right). (F) RNAi knockdown of CG8177 (gray boxes) increases pHi relative to controls (white boxes). n = 17–19 germaria; n = 3 independent replicates. Bars, 10 µm. *, P < 0.05; **, P < 0.01; ***, P < 0.001. P-values were determined with a t test in A and F and a χ² test in B.