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. 2016 Nov 7;215(3):335–344. doi: 10.1083/jcb.201608025

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© 2016 Xue and Sokac

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Figure 2. — Constriction phases correlate with distinct dynamics of ring components. (A–H) Fixed wild-type embryos. Gray shading highlights phase 1. (A, C, E, and G) Rings in cross section at increasing furrow lengths, stained for F-actin (phalloidin; A), Myosin-2 (C), Pnut (E), and Mbs (G). Arrowheads show ring position at furrow tips. Bars, 10 µm. (B, D, F, and H) Fluorescence intensity (arbitrary units [AU]) versus furrow length for F-actin (B), Myosin-2 (D), Pnut (F), and Mbs (H; n ≥ 43 embryos per staining and 10 ring profiles per embryo). Splines show trend of change for data points. Clouds show one SD from the moving mean.