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. 2016 Nov 8;15:539. doi: 10.1186/s12936-016-1584-z

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Bland-Altman plots to assess agreement between light microscopy, QT-NASBA and qRT-PCR levels in samples from naturally infected individuals. In the y-axes, differences between gametocyte levels estimated by two different methods are presented: a QT-NASBA levels minus microscopy-defined densities, b qRT-PCR minus microscopy levels, c qRT-PCR levels minus levels estimated by QT-NASBA. The X-axes present the average of the densities estimated by the two methods included in the calculation of the respective Y-axes. In panel a, the differences (Y-axis) are limited to a narrower range of values compared to panels b and c, and the inset plot presents the same data using different Y-axis limits