Fig. 2.

Alignment of HsNek2/Pfnek-2 protein sequences and the viability of parasite cultures following exposure to NCL-00016066. a Alignment of the amino acid sequences of Hsnek2 and Pfnek-2. The conserved methionine gatekeeper (magenta) and the conserved phenylalanine (green) are shown, as are the conserved HRD sequence (yellow) with the conserved activation loop motifs DFG and APE/SPE (turquoise). The valine at position 24 in Pfnek-2 is a cysteine in Hsnek2 and is highlighted in red. The identical amino acids between Hsnek-2 and Pfnek-2 are indicated with a asterisk (*), the highly conserved amino acids with two dots (:) and the conserved amino acids with one dot (.). b, c The viability of parasites in the presence of varying concentrations of NCL-00016066 as measured by (b) SYBR Green I and (c) LDH assays. As the parasites are chloroquine sensitive, chloroquine (20 μM) was included as a positive control while non-infected red blood cells, without inhibitor, and 10% DMSO were included as negative controls. The results are presented as the mean ± SEM. (n = 3)