Fig. 3.

Phosphorylation of in vitro protein kinase substrates by Pfnek-2 and Pfnek-2(V24C). a, b Phosphorylation of the exogenous substrates myelin basic protein (MBP) and α-casein by Pfnek-2 and Pfnek-2(V24C). Shown is a Coomassie blue stained radioactive gel (a) and the resulting autoradiograph (b), which are representative of three experiments. The position of molecular weight markers (kDa) are shown