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. 2016 Nov 7;15:535. doi: 10.1186/s12936-016-1580-3

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Irreversible inhibition of Pfnek-2(V24C) activity by NCL-00016066. a, b Activity of NCL-00016066 (20 μM) against Pfnek-2 (a) and Pfnek-2(V24C) (b) before and after wash out of the inhibitor. Shown are the autoradiographs (a, b). The position of molecular weight markers (kDa) are shown. c Quantification of three independent experiments indicating the effect of washing on Pfnek-2(V24C). Data shown is the mean ± S.E.M; n = 3. Data analysed using Students paired t-test *p ≤ 0.05