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. 2016 Nov 8;14:16. doi: 10.1186/s12948-016-0053-0

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — CHI3L1 protein is secreted by Th17 and non-classic Th1 cells. a CHI3L1 protein (40 kDa) expression was evaluated on cell lysates of Th17, non classic-Th1 and classic-Th1 clones by western blot analysis. b Secreted CHI3L1 was measured on cell culture supernatants of Th17 (n = 7), non classic Th1 (n = 7) and classic Th1 (n = 7) clones after 72 h stimulation with medium (white columns), anti-CD3/CD28 mAbs (grey columns) or PMA plus Ionomycin (black columns) by ELISA. Columns represent mean values (±SE). *p ≤ 0.05