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Averaged transformation efficiencies of the S. islandicus ΔIII-Bα and IIIB-β deletion mutant after challenging with plasmid-borne L2S1 protospacers carrying all possible dinucleotide modifications of the CCN PAM sequence. Transformation efficiency of the non-target plasmid is set at 100% and error bars derive from triplet experiments for each construct. Transformation efficiency and standard deviation values for the wild-type and mutated strains are listed in Table S2.
