Table 1.

Protospacer L2S1 carrying multiple base pair mismatches at positions 1 to 10. Averaged transformation efficiencies (TE) from triplicate experiments were normalized to values for the control plasmid constructs set to 100%. The sizes of CRISPR deletions detected in surviving transformants are shown with the repeat (r) extremities. 0 - no deletion detected. n.d. not determined. The major annealing site is centered on the shaded positions.

Construct	1A	2C	3A	4C	5T	6G	7G	8G	9C	10G	TE (%)	CRISPR deletion
1	C	T									< 0.1	r1-r63
2	C	A	G								< 0.1	r1-r63
3	C	T	G								0.1	r1-r80
4	C	T	G	T							0.2	r1-r70
5	C	T	G	G							1.1	r1-r80
6	C	T	G	A							13	0
7	T	G	T								< 0.1	n.d.
8	T	G	T	G							12	0
9	C	T	G	A	C						17	0
10	T	G	T	G	A						24	0
11		G	T	G	A						9	0
12		G	T	G	A	C					17	0
13			T	G	A	C	C				79	0
14				G	A	C					10	0
15					A	C	C	C			0.1	n.d.
16						C	C	C	G	C	< 0.1	n.d.
17							C	C	G		< 0.1	n.d.