Table 2.
Protospacer L2S1 carrying multiple base pair mismatches at positions 19 to 27. Averaged transformation efficiencies (TE) from triplicate experiments were normalized to values for control plasmid constructs set to 100%. The sizes of CRISPR deletions in surviving transformants are given with repeat (r) extremities. n.d. - not determined. The minor annealing site is centered on the shaded positions.
| Construct | 19A | 20A | 21C | 22G | 23A | 24C | 25A | 26G | 27G | TE (%) | CRISPR deletion |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | T | T | G | 0.1 | n.d. | ||||||
| 2 | T | T | G | C | T | 1 | r1-r7 | ||||
| 3 | T | G | C | T | G | T | C | 10 | n.d. | ||
| 4 | G | C | T | G | T | 6 | n.d. | ||||
| 5 | C | T | G | T | 1.1 | r1-r26 | |||||
| 6 | C | T | G | 1 | r1-r7 | ||||||
| 7 | T | G | T | C | 1.2 | n.d. | |||||
| 8 | C | T | < 0.1 | n.d. | |||||||
| 9 | T | G | 0 | n.d. | |||||||
| 10 | C | G | 0 | n.d. | |||||||
| 11 | T | C | C | < 0.1 | n.d. |