Table 3.

Normalized average transformation efficiencies obtained with plasmid-borne protospacers matching 5 different spacers of CRISPR loci 1 and 2. The constructs carry quintipulet mismatches with transversions distributed along the protospacer. Experiments were performed in triplicate. Results for the major and minor annealing sites are shaded.

	CRISPR spacer – TE% values
Mutated quintuplet	L2-S1 (61.5% GC)	L1-S58 (61.5% GC)	L2-S55 (60.5% GC)	L2-S45 (49% GC)	L1-S61 (25.6% GC)
3 – 7	79 (±7.1)	87.3 (±19.9)	66.3 (± 11.2)	41.5 (± 11.5)	25 (± 2.5)
11 – 15	0.031 (±0.41)	0.17 (±0.12)	0. 1 (± 0. 1)	0.2 ( ± 0.1)	0.16 (± 0.14)
16 – 20	0.002 (±0.002)	0.16 (±0.05)	0.1 (± 0.04)	0.01 (± 0.01)	0.04 (± 0.01)
21 – 25	6 (±4.2)	7.4 (± 5.7)	3.4 (± 1.7)	1.05 (± 0.05)	0.8 (± 0.1)
31 – 35	0.023 (±0.032)	0.6 (±0.2)	0.7 (±0.3)	0. 3 (± 0.03)	0.44 (± 0.07)