Drosophila egg chamber and drongo gene model. (A) Model system used for these studies; stage 8 Drosophila egg chamber: oocyte, nurse cells, follicle cells, and ring canals (arrows). A = anterior, P = posterior. (B) RT-PCR results for full length drongo transcripts using total RNA isolated from wild type ovaries. Expected sizes for drongo-RI and drongo-RH transcripts are 2,625 and 3,518 nucleotides, respectively. (C) drongo gene model (FlyBase: Gbrowse): boxes highlight exons, orange for coding regions and gray for UTRs. On the 2L chromosome fragment (black ruler), black arrows indicate P-element insertion location in the drongo gene. Red arrows indicate the regions targeted by drongo-specific molecular beacons.