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. 2016 Aug 11;13(11):1117–1132. doi: 10.1080/15476286.2016.1218592

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Figure 2. — drongo mRNA localization in fixed and live oocytes using drongo-specific molecular beacons. (A) Detection of endogenous drongo transcript using dH1598-AF647 in fixed, wild type oocytes. drongo mRNA localization is indicated in the germline (arrows) and soma (asterisks). Microinjection of dH1598-AF647 in a nurse cell of a (B) wild type (XY-projection of 9 Z-slices/1 μm each) or (B’) GFP-Stau/CyO (XY-projection of 12 Z-slices/1 μm each) egg chamber at the indicated time points. Microinjection of dH1598-AF647 into a nurse cell of a (C) stage 7–8 (XY-projection of 5 Z-slices/1 μm each) or (C’) stage 10A (XY-projection of 5 Z-slices/1 μm each) Me31B-YFP egg chamber at the indicated time points. (D) Microinjection of dH1598-AF647 in a nurse cell of an armi mutant egg chamber (XY-projection of 10 Z-slices/1 μm each) at the indicated time points. s = stage. Bars, 20 μm.