Figure 6.
Colocalization of Drongo with actin related proteins, or small GTPases at stages 8–9 of oogenesis. (A) Drongo-EGFP and Arpc3A in a stage 8–9 egg chamber. (B) Drongo-EGFP and Rab11 (yellow: colocalization as XY-projection of 61 Z-slices/0.5 μm each, shown as an overlayer on the 27th Z-slice), in a MTD>drongoEGFP stage 8–9 oocyte. F-actin was highlighted using fluorescently labeled phalloidin. (C) Drongo and Arf51F-GFP in a stage 8–9 egg chamber expressing endogenous GFP-tagged Arf51F under native promoter control. (A-C) Colocalization at the oocyte's cortex shown in a normalized fluorescence intensity profile measured along the lines drawn as shown in the merged panels. Arrows indicate colocalization at the cortex of the oocyte. A = anterior, P = posterior. Bars, 20 μm.