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. 2016 Nov 8;7:1769. doi: 10.3389/fmicb.2016.01769

Table 2.

Primers used for the identification of lactic acid bacteria (LAB) by multiplex PCR.

Primer Sequence Target
Primer mixture I
SCAR-OENI-F GGTAGATTAACCCGCGACG O. oeni
SCAR-OENI-R GGAATCGGTAGCATCCTG
SCAR-LBR-F GGAAGATCAAGAATATCGGTG L. brevis
SCAR-LBR-R GCGTCTCTAATTCACTGAGC
SCAR-LPL-F GAAGATTTGCCCATCGGTG L. plantarum
SCAR-LPL-R CGTTTGATGGTAGCGTTGC
SCAR-LEU-F GTGGTCATGGGTCTTAGC Leuconostoc
SCAR-LEU-R GGATCAAGACTAGCCAATGG mesenteroides
SCAR-WPA-F GCTGATGAACCCATACCTC Weissella paramesenteroides
SCAR-WPA-R GACCTGATTCGCTCGTTG
SCAR-PDA-F GTCTAAACTGGTGGTTAAACG P. damnosus
SCAR-PDA-R ATCGCACCTGGTTCAATGC
SCAR-PPA-F GCATGAATCACTTTTCGCTC P. parvulus
SCAR-PPA-R CAAAGATTGTGACCCAGTTG
Primer mixture II
SCAR-LBU-F CTATCTTTAACCGCATTGCCG L. buchneri
SCAR-LBU-R GACACGCTTCTCATGATTGTC
SCAR-PAC-F ATGATGGACAGACTCCCTG P. acidilactici
SCAR-PAC-R CGAGCTGCGTAGATATGTC
SCAR-LBH-F TTCCTTGGTAATGTGCTTGC L. hilgardii
SCAR-LBH-R AATGGCAATCGCAATGGACG
SCAR-PIN-F CTATCCTTACAATGTGCATCG P. inopinatus
SCAR-PIN-R TGGTGCGTCAGTAAATGTAAG
SCAR-LCU-F CCAGATCCATCAGAAGATACG L. curvatus
SCAR-LCU-R GCTAACTTACCACTAACGACC
SCAR-PPE-F GGGAACGGTTTTAGTTTTATACG P. pentosaceus
SCAR-PPE-R CTAAGAGCGGTGATGATAAG