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. 2016 Aug 2;44(19):9296–9314. doi: 10.1093/nar/gkw682

Figure 9.

Figure 9.

Role of Mek1 kinase activity in meiotic recombination of rec8Δ and rec8 phospho-mutant. (A) Schematic representation of experimental procedures (see Materials and Methods). (B) Representative images of 2D-gels of rec8Δ mek1as in the absence or presence of 1-NA-PP1 at the indicated time points. Square brackets and arrowheads denote JMs and DSBs, respectively. (C) Quantification of recombination intermediates in the rec8Δ mek1as strain shown in (B). (D) Representative images of 2D-gels of rec8-29A mek1as in the absence or presence of 1-NA-PP1 at the indicated time points. Square brackets and arrowheads denote JMs and DSBs, respectively. (E) Quantification of recombination intermediates in the rec8-29A mek1as strain shown in (D).