Figure 3.

Sequencing errors and rare variants generate bubbles in DBGs with very unbalanced path coverage. (A) For ease of exposition of the concept, we represent here the reads mapping to a reference genome. Applying an absolute cutoff would remove the sequencing error for a poorly expressed gene, but not for a highly expressed gene. (B) Applying a relative cutoff of 5% in the DBG removes one or two edges from the red path and hence prevents this bubble from being found.