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. 2016 Jul 25;44(19):e147. doi: 10.1093/nar/gkw654

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — An example of correctly extracted and sheared DNA visualized using field inversion gel electrophoresis. The ladder is the NEB low range PFG marker (no longer produced). The lanes of the gel are as follows: (A) ladder, (B) unsheared DNA, (C) DNA sheared with a 24 gauge needle, (D) sheared DNA size selected with 15–50 kb cut-off, (E) SMRTbell template library after 15–50 kb size selection. From the gel, it is evident that there is a minimal ‘tail’ of DNA below ∼15 kb, the preferred size selection minimum.