Figure 1.

Scd6 gets arginine methylated in vivo. (A) Galactose-inducible Scd6 and its RGG-deletion variant was pulled down from wild-type yeast cells. The blots were probed with mono-methyl arginine (MMA) specific antibody and Peroxidase-anti peroxidase (PAP) antibody, in that order after stripping the blot. * Represents another arginine-methylated protein. (B) Quantitation of three independent experiments (n = 3) that were performed as in A. (C) Galactose-inducible His-Scd6 was pulled down from wild-type and Δhmt1 cells followed by probing as explained in A. * Represents another arginine-methylated protein. (D) Quantitation of three independent experiments (n = 3) that were performed as mentioned in C.