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. 2016 Sep 8;44(19):9358–9368. doi: 10.1093/nar/gkw762

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Arginine methylation defective (AMD) mutant of Scd6. (A) Arginines (green) mutated to alanine in different mutants have been shown. Three, five, eight and nine arginines were mutated in Mut1, Mut2, Mut3 and AMD mutant, respectively. Only the RGG-motif sequence (283–349) of Scd6 has been shown. (B) His pull-down was performed from cells expressing galactose-induced mutants. Western analysis was performed with MMA antibody followed by probing with Peroxidase anti-peroxidase (PAP). (C) Quantitation of three independent experiments (n = 3) that were performed as described in B. (D) Expression level analysis of AMD mutant in yeast cells using PAP antibody.