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. 2016 Sep 8;44(19):9358–9368. doi: 10.1093/nar/gkw762

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — AMD mutant of Scd6 is defective in repressing translation. (A) AMD mutant rescues growth defect caused by overexpression of SCD6. Five independent growth assay experiments (n = 5) were performed. (B) Live cell imaging of stress granule formation upon overexpression of wild-type and AMD mutant in Pab1-GFP strain. (C) Quantitation of Pab1-GFP granule formation by AMD mutant. Three independent experiments (n = 3) were considered for quantitation. (D) Western analysis to compare Pab1-GFP levels in cell lysates from strains expressing wild-type Scd6 and AMD mutant. Blots were probed with anti-GFP (upper panel) followed by probing with anti-PGK1 (lower panel).