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. 2016 Sep 8;44(19):9358–9368. doi: 10.1093/nar/gkw762

Figure 6.

Figure 6.

AMD mutant is defective in inducing eIF4G1-granule formation. (A) Live-cell imaging of eIF4G1-GFP strain upon overexpression of wild-type and AMD mutant of Scd6. (B) Quantitation of eIF4G1-GFP granule formation. Three independent experiments (n = 3) were considered for quantitation. (C) Western analysis to compare eIF4G1-GFP levels in cell lysates from strains expressing wild-type Scd6 and AMD mutant. Blots were probed with anti-eIF4G1 (upper panel) followed by probing with anti-PGK1 (lower panel).