Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jul 25;44(19):e149. doi: 10.1093/nar/gkw660

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 2. — A one-step method to generate a multiple sgRNA delivery plasmid. (A) Schematic representation of the Lenti-multi-Guide and Lenti-multi-CRISPR plasmids. LTR, long terminal repeat; U6, U6 promoter; Lac, Lac promoter; Cm^r, Chloramphenicol resistance; ccdB, toxin ccdB gene; sg Scaffold, sgRNA scaffold sequence; EF1a, EF1α promoter; Cas9, spCas9; Puro, puromycin resistance; WPRE, Woodchuck hepatitis virus post-transcriptional regulatory element. Retroviral Ψ packaging element and Rev response element were not shown. (B) Overview of the cloning strategy.