Figure 6.
Panel (A) M.DbaKI-mut1 methylated plasmid expressing the M.DbaKI-mut1 MTase is resistant to BglI digestion, even in the same reaction in which a control unmodified DNA (pBR322) is cut. The M.DbaKI-mut1 expression plasmid has BglI recognition sites, as indicated by BglI cleavage of a PCR amplicon, which erases the M.DbaKI-mut1 modification of the plasmid. Arrows indicate the PCR primer positions. Resistance to BglI digestion confirms the SMRT sequence methylome analysis shown in Panel B that demonstrates M.DbaKI-mut1 produces Gm4CC-5-GGC modification.