Table 2. Restriction of λvir by R-M systems cloned in E. coli.
| Host | eop (efficiency of plating) | Restriction |
|---|---|---|
| A. Naive phage (1st passage: M-) | ||
| ER2796_RM- | 1 | – |
| ER2796_DbaKI_RM+ | 0.03 | 30x |
| ER2796_Apa12260I_RM+ | 0.04 | 25x |
| B. Phage isolated from restricting host (2nd passage: M+) | ||
| ER2796_RM- | 1 | – |
| ER2796_DbaKI_RM+ | 0.7 | 1.5x |
| ER2796_RM- | 1 | – |
| ER2796_Apa12260I_RM+ | 0.3 | 3x |
| C. Phage from 2nd passage isolated from non-restricting host (3rd passage: M-) | ||
| ER2796_RM- | 1 | – |
| ER2796_DbaKI_RM+ | 0.004 | 250x |
| ER2796_RM- | 1 | – |
| ER2796_Apa12260I_RM+ | 0.007 | 130x |
eop: average of 3 plates in 2 replicates.
sites: DbaKI and Apa12260I each have 8 recognition sites in Lambdavir.