. 2016 Aug 31;44(19):9413–9425. doi: 10.1093/nar/gkw743

Table 3. Modification detection by SMRT sequencing.

`Enzyme`	`MTase construct`	`m6A%detected`	`m4C%detected`	`Coverage`
`PacII`		`RCAAY`	`CCC`
	`Native organism`	`95.4`	`84.5`	`41X`
	`M1 + M2 + S`	`93.7`	`63.0`	`30x`
	`M1-APPA + M2 + S`	`–`	`56.6`	`43x`
	`M1 + M2-APPA + S`	`90.7`	`24.9`	`36x`
	`M1 + S`	`61.7`	`–`	`81x`
	`M2 + S`	`–`	`–`	`90x`
`BceNI`		`GAG`	`CCC`
	`Native organism`	`97.4`	`66.4`	`38x`
	`M1 + M2 + S`	`98.6`	`90.2`	`49x`
	`M1-APPA + M2 + S`	`–`	`45.5`	`60x`
	`M1 + M2-APPA + S`	`82.3`	`–`	`52x`
	`M1 + S`	`46.6`	`–`	`40x`
	`M2 + S`	`–`	`–`	`90x`
`Mma5219I`		`GCA`	`TCY`
	`Native organism`	`94.0`	`72.4`	`42x`
	`M1 + M2 + S`	`73.4`	`33.0`	`26x`
	`M1-APPA + M2 + S`	`–`	`–`	`29x`
	`M1 + M2-APPA + S`	`89.6`	`–`	`51x`
	`M1 + S`	`80.2`	`–`	`40x`
	`M2 + S`	`–`	`–`	`65x`
`DbaKI`		`GAAG`	`GCC`
	`Native organism`	`91.9`	`68.8`	`29x`
	`M1 + M2 + S`	`100`	`97.7`	`46x`
	`M1-APPA + M2 + S`	`–`	`100`	`144x`
	`M1 + M2-APPA + S`	`100`	`98.7`	`99x`
	`M1 + S`	`35.9`	`–`	`60x`
	`M2 + S`	`–`	`–`	`51x`

M-APPA indicates catalytic mutant (NPPF or NPPY to APPA) of the indicated MTase subunit.