Table 4. Hybrid all-m4C systems: modification detection by SMRT sequencing.
| Enzyme | MTase construct | Motif / m4C%detected | Coverage | |
|---|---|---|---|---|
| PacII-PacII S hybrid: CCC-5-GGG | CCC | |||
| M1 + M2 | 68.5 | 42x | ||
| M1-APPA + M2 | 45.1 | 43x | ||
| M1 + M2-APPA | – | 36x | ||
| M1 + -- | – | 81x | ||
| -- + M2 | – | 90x | ||
| PacII-Mma5219I S hybrid: CCC-5-RGA | CCC | TCY | ||
| M1 + M2 | 68.9 | 67.1 | 39x | |
| M1 + -- | – | – | 81x | |
| -- + M2 | – | – | 90x | |
| PacII-Asu14238II S hybrid: CCC-5-GGC | CCC | GCC | ||
| M1 + M2 | 48.6 | 63.8 | 43x | |
| M1 + -- | – | – | 42x | |
| -- + M2 | – | – | 46x | |
| DbaKI-DbaKI S hybrid: GCC-5-GGC | GCC | |||
| M1 + M2 | 83.3 | 100x | ||
| M1 + -- | – | 46x | ||
| -- + M2 | – | 27x | ||
| DbaKI-Apa12260I S hybrid: GCC_N5-GGC | GCC | |||
| M1 + M2 | 84.9 | 98x | ||
| M1 + -- | – | 34x | ||
| -- + M2 | – | 28x | ||
| DbaKI-Asu14238II S hybrid: GCC_N5-GGC | GCC | |||
| M1 + M2 | 86.7 | 100x | ||
| M1 + -- | – | 32x | ||
| -- + M2 | – | 27x | ||
M-APPA indicates catalytic mutant (NPPF or NPPY to APPA) of the indicated MTase subunit.